ISOLASI KANDIDAT BAKTERI PROBIOTIK DARI USUS IKAN KAKAP PUTIH (LATES CALCARIFER) BERDASARKAN UJI AKTIVITAS ANTIBAKTERI SECARA IN VITRO

Abstract

White sea bass (Lates calcarifer) is a fish that has a high commodity and high selling value. White sea bass (Lates calcarifer) has a market demand that continues to increase every year. The fish farming process is hampered by disease attacks, one of which is vibriosis which is caused by vibrios. Treatment efforts to overcome these pathogenic infections are by using antibiotics which cause pathogenic bacteria to become resistant. The drug-based controls that have been widely implemented so far have backfired on the export of cultivated products and have resulted in a ban by the government. Therefore, it is necessary to work around this by exploring candidate probiotic bacteria in the intestines of white snapper (Lates calcarifer) which can be used as a substitute for the use of antibiotics. The research began by isolating bacteria from the intestines of snapper fish, then characterization of the isolates morphologically and physiologically, and antibacterial testing against Vibrio sp. In this study, 16 isolates were obtained with morphological characteristics, namely round-shaped colonies, cream color, dark cream, convex and flat elevations, entire edges, moderate and small sizes. 15 of the 16 colonies were Gram Positive bacteria which were characterized by maintaining a violet color. As for the antibacterial activity test results, there was the highest inhibition zone in chloramphenicol with an average diameter of mm (13.6 ± 0.10b) in the strong category and in K.P6.3 with an average diameter of mm (9 ± 0.6ab). So it can be concluded that isolate K.P6.3 has results that are not significantly different from the antibiotic chloramphenicol.