ISOLASI BAKTERI DARI ORGAN EKOR LOBSTER BATU ( PANULIRUS PENICILLATUS )

Abstract

Lobster is an organism that has very high economic value. The high economic value of lobster makes fishermen and cultivators try to balance production and existing market needs. There is a decline in production in lobster cultivation and wild catches, failures often occur due to high mortality caused by disease infections in lobsters which are generally caused by bacterial, parasitic, fungal and viral infections. This research aims to isolate and identify bacteria from the tail organs of rock lobsters to inhibit pathogens such as Escheria coli and Pseudomonas Aeruginosa. Bacterial isolation was carried out by diluting the sample from 10-1,10-3,10-5, the three dilutions were cultured and isolated using the spread method on TSA (Tryptic Soy Agar) media, then incubated for 24 hours at room temperature. Next, the bacteria that have grown are purified until pure cultures with the same morphology are obtained on new TSA media. After the pure culture grows on TSA media, morphological observations are carried out to determine the color, shape, elevation, edges and size of the growing isolate. Next, bacterial isolates were transferred from TSA to MRS agar to determine whether the bacteria obtained could grow on MRS agar media. Then a gram stain is carried out to find out whether the bacteria are gram positive or negative. The results from isolation to gram staining showed that bacterial isolates at a concentration of 10-3 were cream colored, round in shape, had flat elevations and flat edges and were small in size. Meanwhile, at a concentration of 10-5, the results were white, round in shape, had flat elevations and flat edges and were small in size.